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Image Search Results
Journal: bioRxiv
Article Title: IRF8-mutant B cell lymphoma evades immunity through a CD74-dependent deregulation of antigen processing and presentation in MHC CII complexes
doi: 10.1101/2023.10.14.560755
Figure Lengend Snippet: ELISA-based granzyme quantification in conditioned media of CD8 OT-I cells co-cultured with mouse B cell lymphoma cell lines A20, BCL1 and 2PK-3, each IRF8 WT or KO, “loaded” or not with OVA. Bottom panels . FACS-based quantification of 7AAD+ lymphoma cells (A20, BCL1, or 2PK-3), IRF8 WT or KO, “loaded” or not with OVA. Data are mean ±SD of three biological replicates, performed with one, two or three technical replicates. P values are from one-way ANOVA with Bonferroni post-test;, *(p<0.05), **(p<0.01), ***(p<0.001), **** (p<0.0001).
Article Snippet: Conditioned media from co-culture of OT-I activated CD8+ T cells with mouse B cell lymphoma (OVAp pulsed and non-pulsed controls) were assessed for the concentrations of Granzyme B by ELISA using
Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture
Journal: Fems Microbiology Letters
Article Title: Regulation of Cu(I)/Ag(I) efflux genes in Escherichia coli by the sensor kinase CusS
doi: 10.1111/j.1574-6968.2012.02529.x
Figure Lengend Snippet: Data shows the relative expression from the cusC gene +/− SEM, as determined in E. coli wild-type (gray), E. coli ΔcusS (checkered) and E. coli ΔcusS/pBADcusS (diagonal lines) after exposure to 5 µM AgNO3 for 0, 2 and 4 hours. Total RNA from cells was extracted, reverse transcribed and cDNA was subjected to real-time PCR using primers specific for cusC. ANOVA analysis with multiple comparisons showed that transcription from cusC is absent when cusS is disrupted (P < 0.0001).
Article Snippet: First strand cDNA was prepared from 2 µg of total RNA using the
Techniques: Expressing, Real-time Polymerase Chain Reaction